Glycine Potentiates AMPA Receptor Function through Metabotropic Activation of GIuN2A-Containing NMDA Receptors
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Authors
Li, Li-Jun
Hu, Rong
Lujan, Brendan J.
Chen, Juan
Zhang, Jian-Jian
Nakano, Yasuko
Cui, Tian-Yuan
Liao, Ming-Xia
Chen, Jin-Cao
Man, Heng-Ye
Issue Date
2016
Type
Article
Language
Keywords
glycine , AMPA receptor , NMDA receptor , ERK1/2 , hippocampal neurons , HEK293 cells
Alternative Title
Abstract
NMDA receptors are Ca2+.-permeable ion channels. The activation of NMDA receptors requires agonist glutamate and co-agonist glycine. Recent evidence indicates that NMDA receptor also has metabotropic function. Here we report that in cultured mouse hippocampal neurons, glycine increases AMPA receptor -mediated currents independent of the channel activity of NMDA receptors and the activation of glycine receptors. The potentiation of AMPA receptor function by glycine is antagonized by the inhibition of ERK1/2. In the hippocampal neurons and in the HEK293 cells transfected with different combinations of NMDA receptors, glycine preferentially acts on GIuN2A-containing NMDA receptors (GIuN2ARs), but not GIuN2B-containing NMDA receptors (GIuN2BRs), to enhance ERK1/2 phosphorylation independent of the channel activity of GIuN2ARs. Without requiring the channel activity of GIuN2ARs, glycine increases AMPA receptor -mediated currents through GIuN2ARs. Thus, these results reveal a metabotropic function of GIuN2ARs in mediating glycine-induced potentiation of AMPA receptor function via ERK1/2 activation.
Description
Citation
Li L-J, Hu R, Lujan B, Chen J, Zhang J-J, Nakano Y, Cui T-Y, Liao M-X, Chen J-C, Man H-Y, Feng H and Wan Q (2016) Glycine Potentiates AMPA Receptor Function through Metabotropic Activation of GluN2A-Containing NMDA Receptors. Front. Mol. Neurosci. 9:102. doi: 10.3389/fnmol.2016.00102
Publisher
License
Creative Commons Attribution 4.0 International
Journal
Volume
Issue
PubMed ID
ISSN
1662-5099