Inter-laboratory optimization of protein extraction method for the assessment of rice endogenous allergens
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Authors
Satoh, Rie
Kitta, Kazumi
Labory-Carcenac, Bénédicte
Lang, Gang-Hua
Schegg, Kathleen
Blumenthal, Kenneth
Hicks, Leslie
Rouquié, David
Herman, Rod A.
Herouet-Guicheney, Corinne
Issue Date
2015
Type
Article
Language
Keywords
rice grain , allergen , proteomics , multiplex immunodetection , two-dimensional difference gel electrophoresis (2D-DIGE)
Alternative Title
Abstract
In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52�"63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ringtrial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an interlaboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14�"16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.
Description
Citation
Satoh, R., Teshima, R., Kitta, K., Lang, G.-H., Schegg, K., Blumenthal, K., … Rascle, J.-B. (2016). Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens. Bioscience, Biotechnology, and Biochemistry, 80(11), 2198�"2207. doi:10.1080/09168451.2016.1206810
Publisher
License
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Journal
Volume
Issue
PubMed ID
ISSN
0105-4538