Isolation and Characterization of an Isoprenoid Transporter Protein in Manduca sexta
Loading...
Authors
Liang, Anna
Issue Date
2015
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Metabolic regulation and transport is a fundamental process in all eukaryotic organisms. Metabolites synthesized throughout the cell must be used in various locations to ensure proper cell function. Isoprenoids are metabolite molecules implicated in cell growth, energy production, and act as precursor to steroids, cholesterol, and other derived compounds in living organisms. Within the isoprenoid pathway, isopentenyl pyrophosphate (IPP) was found to be transported by a specific membrane protein molecule in plants (Bick and Lange, 2003). To date, inhibition of this pathway is used in mammalian drug therapy where isoprenoid utilizing enzymes are the candidates of inhibition accomplished by a class of drugs called stains, though many fundamental properties of this pathway await elucidation. The identification and characterization of an IPP transport protein in Manduca sexta would provide a model system for the study of IPP transport in animals as well as to allow characterization of mitochondrial metabolically derived compounds, which have not been possible to study. By isolating mitochondria from M. sexta, this study aims to purify the unknown transport protein for detailed characterization of isoprenoid pathway. These results may allow inhibition and manipulation of the isoprenoid pathway in insects possibly allowing the discovery of new insecticides.
Description
The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint.
Citation
Publisher
License
In Copyright(All Rights Reserved)