Isolation of Juvenile Hormone Epoxide Hydrolase Genes in the Malpighian Tubules of the Tobacco Hornworm, Manduca Sexta
Loading...
Authors
Fiannaca, Alexander J.
Issue Date
2011
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Understanding the regulation of Juvenile Hormone (JH) titer in insects is critical for understanding the biochemical processes related to metamorphosis. The cDNAs from several potential isoforms of the enzyme Juvenile Hormone Epoxide Hydrolase have been isolated from the Malpighian tubules of Manduca sexta. Modified PCR methods are being used to search for any other potential isoforms of this enzyme that may be contained in a Malpighian tubule expressed sequence tag library. To date, this protocol has yielded three potential genes, in addition to the two originally isolated. Future research will allow the creation of baculovirus vectors containing the isolated potential JHEH genes and the identified isoforms will be expressed in an insect cell culture. The isoforms will be assayed for activity in metabolizing the three forms of JH present in M. sexta through the use of radiolabeled JH’s.
Description
The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint.
Citation
Publisher
License
In Copyright(All Rights Reserved)