Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration
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Authors
Abbas, Syed
Sulharia, Amaad
Clary, Wesley
Del Bianco, Marcus
Guillen, Dario
Harder, Tanaka
Henrique Israel, Luis
Lopez, Joel
McMahon, Adam M.
Schmidt, Monica
Issue Date
2015
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Novel process designs were created in an effort to optimize the efficiency of monoclonal
antibody separation and purification for an industrial process. A base case was
established that utilized common protein separation techniques such as cation exchange
chromatography, anion exchange chromatography, and hydrophobic interaction
chromatography (HIC). Alternate process designs were also made that utilized
immobilized metal affinity chromatography (IMAC) and various multimodal
chromatography mediums. Experiments were conducted to determine the most effective
mediums that could be used in the process design. To better analyze the new alternate
plans, an economic analysis was conducted which determined what alternate plan would
be more cost effective. From analysis, alternate design 1 and alternate design 2a were
determined to be the preferred design due to enhanced performance and lesser overall
capital cost.
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In Copyright(All Rights Reserved)