Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments
Loading...
Authors
Cheng, Qingsu
Aravind, Ashwin
Buckley, Matthew
Gifford, Andrew
Parvin, Bahram
Issue Date
2015
Type
Article
Language
en_US
Keywords
Alternative Title
Abstract
To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.
Description
Citation
Cheng, Q., Aravind, A., Buckley, M., Gifford, A., & Parvin, B. (2015). Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments. Scientific Reports, 5(1). doi:10.1038/srep13685
Publisher
Scientific Reports
License
Attribution 4.0 International
Journal
Volume
Issue
PubMed ID
ISSN
2045-2322