Microparticulate beta-glucan vaccine conjugates phagocytized by dendritic cells activate both naive CD4 and CD8 T cells in vitro
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Authors
Berner, Vanessa K.
DuPré, Sally A.
Redelman, Doug
Hunter, Kenneth W.
Issue Date
2015
Type
Article
Language
en_US
Keywords
Dendritic cells , CD4 T cells , CD8 T cells , Vaccine adjuvant , beta-Glucan
Alternative Title
Abstract
Microparticulate beta-glucan (MG) conjugated to vaccine antigen has been shown to serve as an effective adjuvant in vivo. To further study antigen presentation by MG: vaccine conjugates, bone marrow-derived dendritic cells (BMDC) were treated with MG conjugated to ovalbumin (OVA), then interacted with splenocytes from DO11.10 transgenic mice expressing an OVA peptide-specific T cell receptor. BMDC treated with MG: OVA induced significantly higher numbers of activated (CD25+CD69+) OVA-specific CD4+ T cells than BMDC treated with OVA alone. BMDC treated with MG: OVA upregulated CD86 and CD40 expression as well as MG alone, indicating that conjugation of OVA does not alter the immunostimulatory capacity of MG. Activation of CD8+ OVA-specific OT-1 cells showed that MG: OVA is also capable of enhancing cross-presentation by BMDC to CD8+ cytotoxic T cells. These results show that MG acts as an adjuvant to enhance antigen presentation by dendritic cells to naive, antigen-specific CD4 and CD8 T cells. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Description
Citation
Berner, V. K., duPre, S. A., Redelman, D., & Hunter, K. W. (2015). Microparticulate β-glucan vaccine conjugates phagocytized by dendritic cells activate both naïve CD4 and CD8 T cells in vitro. Cellular Immunology, 298(1-2), 104�"114. doi:10.1016/j.cellimm.2015.10.007
Publisher
Cellular Immunology
License
Attribution-NonCommercial-NoDerivatives 4.0 International
Journal
Volume
Issue
PubMed ID
ISSN
0008-8749