Humoral Immunity Profiling of Subjects with Myalgic Encephalomyelitis Using a Random Peptide Microarray Differentiates Cases from Controls with High Specificity and Sensitivity
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Authors
Singh, Sahajpreet
Stafford, Phillip
Schlauch, Karen A.
Tillett, Richard R.
Gollery, Martin
Johnston, Stephen A.
Khaiboullina, Svetlana F.
De Meirleir, Kenny L.
Rawat, Shanti
Mijatovic, Tatjana
Issue Date
2018
Type
Article
Language
Keywords
Antibody , Chronic fatigue syndrome , Immunosignature , Myalgic encephalomyelitis , Peptide array
Alternative Title
Abstract
Myalgic encephalomyelitis (ME) is a complex, heterogeneous illness of unknown etiology. The search for biomarkers that can delineate cases from controls is one of the most active areas of ME research however, little progress has been made in achieving this goal. In contrast to identifying biomarkers that are directly involved in the pathological process, an immunosignature identifies antibodies raised to proteins expressed during, and potentially involved in, the pathological process. Although these proteins might be unknown, it is possible to detect antibodies that react to these proteins using random peptide arrays. In the present study, we probe a custom 125,000 random 12-mer peptide microarray with sera from 21 ME cases and 21 controls from the USA and Europe and used these data to develop a diagnostic signature. We further used these peptide sequences to potentially uncover the naturally occurring candidate antigens to which these antibodies may specifically react with in vivo. Our analysis revealed a subset of 25 peptides that distinguished cases and controls with high specificity and sensitivity. Additionally, Basic Local Alignment Search Tool (BLAST) searches suggest that these peptides primarily represent human self-antigens and endogenous retroviral sequences and, to a minor extent, viral and bacterial pathogens.
Description
Citation
Singh, S., Stafford, P., Schlauch, K. A., Tillett, R. R., Gollery, M., Johnston, S. A., … Lombardi, V. C. (2016). Humoral Immunity Profiling of Subjects with Myalgic Encephalomyelitis Using a Random Peptide Microarray Differentiates Cases from Controls with High Specificity and Sensitivity. Molecular Neurobiology, 55(1), 633–641. doi:10.1007/s12035-016-0334-0
Publisher
Journal
Volume
Issue
PubMed ID
ISSN
0893-7648