Analysis of Peptide Quantification and Digestion through Assays, HPLC, and Modification of Workflow
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Authors
George, Tyler T.
Issue Date
2015
Type
Thesis
Language
en_US
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Abstract
The goal of the project is to find a believable and accurate method to quantify
peptides, by using a variety of commercial protein assays and HPLC. In the field of
proteomics, mass spectrometry is the premier method for attaining the mass to charge
ratio of a protein. Proteins must be applied to the mass spectrometer as digested peptide
fragments. Quantification of peptide samples is therefore an important topic in both the
preparation of samples to apply, as well as important information of unknown samples.
Currently there is no method by which peptide concentration can be precisely found.
Through the application and modification of already existing protein assays as well as
HPLC methods, the sensitivity of the assay and technology for peptide samples will be
measured. Methods in the workflow leading up to mass spectrometry must be considered
as well such as purification of samples, robustness of methods to solubilize proteins and
the methods by with proteins are digested.
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In Copyright(All Rights Reserved)