Human Galectin-1 Improves Sarcolemma Stability and Muscle Vascularization in the mdx Mouse Model of Duchenne Muscular Dystrophy
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Authors
Wuebbles, Ryan D.
Cruz, Vivian
Van Ry, Pam
Barraza-Flores, Pamela
Brewer, Paul D.
Jones, Peter L.
Burkin, Dean J.
Issue Date
2019
Type
Article
Language
en_US
Keywords
Alternative Title
Abstract
Duchenne muscular dystrophy (DMD) is a devastating disease caused by mutations in the dystrophin gene that result in the complete absence of dystrophin protein. We have shown previously that recombinant mouse Galectin-1 treatment improves physiological and histological outcome measures in the mdx mouse model of DMD. Because recombinant human Galectin-1 (rHsGal1) will be used to treat DMD patients, we performed a dose-ranging study and intraperitoneal or intravenous delivery to determine the efficacy of rHsGal1 to improve preclinical outcome measures in mdx mice. Our studies showed that the optimal dose of rHsGal1 delivered intraperitoneally was 20 mg/kg and that this treatment improved muscle strength, sarcolemma stability, and capillary density in skeletal muscle. We next examined the efficacy of intravenous delivery and found that a dose of 2.5 mg/kg rHsGal1 was well tolerated and improved outcome measures in the mdx mouse model. Our studies identified that intravenous doses of rHsGal1 exceeding 2.5 mg/kg resulted in toxicity, indicating that dosing using this delivery mechanism will need to be carefully monitored. Our results support the idea that rHsGal1 treatment can improve outcome measures in the mdx mouse model and support further development as a potential therapeutic agent for DMD.
Description
Citation
Wuebbles, R. D., Cruz, V., Van Ry, P., Barraza-Flores, P., Brewer, P. D., Jones, P., & Burkin, D. J. (2019). Human Galectin-1 Improves Sarcolemma Stability and Muscle Vascularization in the mdx Mouse Model of Duchenne Muscular Dystrophy. Molecular Therapy - Methods & Clinical Development, 13, 145–153. doi:10.1016/j.omtm.2019.01.004
Publisher
Molecular Therapy - Methods and Clinical Development
Journal
Volume
Issue
PubMed ID
ISSN
2329-0501