Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
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Authors
Gomzikova, Marina O.
Zhuravleva, Margarita N.
Miftakhova, Regina R.
Arkhipova, Svetlana S.
Evtugin, Vladimir G.
Khaiboullina, Svetlana F.
Kiyasov, Andrey P.
Persson, Jenny L.
Mongan, Nigel P.
Pestell, Richard G.
Issue Date
2017
Type
Article
Language
Keywords
extracellular vesicles , membrane vesicles , cytochalasin B-induced membrane vesicles , angiogenesis , cell-free therapy
Alternative Title
Abstract
Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.
Description
Citation
Gomzikova, M. O., Zhuravleva, M. N., Miftakhova, R. R., Arkhipova, S. S., Evtugin, V. G., Khaiboullina, S. F., … Rizvanov, A. A. (2017). Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells. Oncotarget, 8(41). doi:10.18632/oncotarget.19723
Publisher
Journal
Volume
Issue
PubMed ID
ISSN
1949-2553