Kaposi Sarcoma Herpes Virus Latency Associated Nuclear Antigen Protein Release the G2/M Cell Cycle Blocks by Modulating ATM/ATR Mediated Checkpoint Pathway

Loading...
Thumbnail Image

Authors

Kumar, Amit
Sahu, Sushil K.
Mohanty, Suchitra
Chakrabarti, Sudipta
Maji, Santanu
Reddy, Rajendra
Jha, Asutosh K.
Goswami, Chandan
Kundu, Chanakya N.
Rajasubramaniam, Shanmugam

Issue Date

2014

Type

Article

Language

Keywords

Virology

Research Projects

Organizational Units

Journal Issue

Alternative Title

Abstract

The Kaposi's sarcoma-associated herpesvirus infects the human population and maintains latency stage of viral life cycle in a variety of cell types including cells of epithelial, mesenchymal and endothelial origin. The establishment of latent infection by KSHV requires the expression of an unique repertoire of genes among which latency associated nuclear antigen (LANA) plays a critical role in the replication of the viral genome. LANA regulates the transcription of a number of viral and cellular genes essential for the survival of the virus in the host cell. The present study demonstrates the disruption of the host G2/M cell cycle checkpoint regulation as an associated function of LANA. DNA profile of LANA expressing human B-cells demonstrated the ability of this nuclear antigen in relieving the drug (Nocodazole) induced G2/M checkpoint arrest. Caffeine suppressed nocodazole induced G2/M arrest indicating involvement of the ATM/ATR. Notably, we have also shown the direct interaction of LANA with Chk2, the ATM/ATR signalling effector and is responsible for the release of the G2/M cell cycle block.

Description

Citation

Publisher

Journal

Volume

Issue

PubMed ID

ISSN

EISSN

Collections