A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis
Loading...
Authors
Phokrai, Phornpun
Karoonboonyanan, Wisansanee
Thanapattarapairoj, Nida
Promkong, Chidchanok
Dulsuk, Adul
Koosakulnirand, Sirikamon
Canovali, Sasha
Indrawattana, Nitaya
Jutrakul, Yaowaruk
Wuthiekanun, Vanaporn
Issue Date
2018
Type
Article
Language
Keywords
Burkholderia pseudomallei , immunochromatography test , antibody detection , melioidosis , point-of-care test , rapid test , serological diagnosis
Alternative Title
Abstract
Melioidosis is a fatal infectious disease caused by the environmental bacterium Burkholderia pseudomallei. It is highly endemic in Asia and northern Australia but neglected in many other tropical countries. Melioidosis patients have a wide range of clinical manifestations, and definitive diagnosis requires bacterial culture, which can be time-consuming. A reliable rapid serological tool is greatly needed for disease surveillance and diagnosis. We previously demonstrated by enzyme-linked immunosorbent assay (ELISA) that a hemolysin-coregulated protein (Hcp1) is a promising target for serodiagnosis of melioidosis. In this study, we developed a rapid immunochromatography test (ICT) using Hcp1 as the target antigen (Hcp1ICT). We evaluated this test for specific antibody detection using serum samples obtained from 4 groups of human subjects, including the following: (i) 487 culture-confirmed melioidosis patients from four hospitals in northeast Thailand
(ii) 202 healthy donors from northeast Thailand
(iii) 90 U.S. healthy donors
and (iv) 207 patients infected with other organisms. Compared to culture results as a gold standard, the sensitivity of ICT for all hospitals was 88.3%. The specificities for Thai donors and U.S. donors were 86.1% and 100%, respectively, and the specificity for other infections was 91.8%. The results of the Hcp1-ICT demonstrated 92.4% agreement with the Hcp1-ELISA results with a kappa value of 0.829, indicating that the method is much improved compared with the current serological method, the indirect hemagglutination assay (IHA) (69.5% sensitivity and 67.6% specificity for Thais). The Hcp1-ICT represents a potential point-of-care (POC) test and may be used to replace the IHA for screening of melioidosis in hospitals as well as in resource-limited areas.
(ii) 202 healthy donors from northeast Thailand
(iii) 90 U.S. healthy donors
and (iv) 207 patients infected with other organisms. Compared to culture results as a gold standard, the sensitivity of ICT for all hospitals was 88.3%. The specificities for Thai donors and U.S. donors were 86.1% and 100%, respectively, and the specificity for other infections was 91.8%. The results of the Hcp1-ICT demonstrated 92.4% agreement with the Hcp1-ELISA results with a kappa value of 0.829, indicating that the method is much improved compared with the current serological method, the indirect hemagglutination assay (IHA) (69.5% sensitivity and 67.6% specificity for Thais). The Hcp1-ICT represents a potential point-of-care (POC) test and may be used to replace the IHA for screening of melioidosis in hospitals as well as in resource-limited areas.
Description
Citation
Phokrai, P., Karoonboonyanan, W., Thanapattarapairoj, N., Promkong, C., Dulsuk, A., Koosakulnirand, S., … Chantratita, N. (2018). A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis. Journal of Clinical Microbiology, 56(8). doi:10.1128/jcm.00346-18
Publisher
Journal
Volume
Issue
PubMed ID
ISSN
0095-1137