Inducible Cre-mediated Excision Yields Incomplete Ano1 Knockdown in the Murine Gastrointestinal Tract
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Authors
Lopez, Justin
Issue Date
2013
Type
Thesis
Language
en_US
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Abstract
Anoctamin-1 (ANO1) is a calcium-activated chloride channel that is highly expressed in
the muscle layer of the murine gastrointestinal (GI) tract, particularly interstitial cells of
Cajal (ICC) responsible for regulation of GI contraction. The particular function of
ANO1 in the GI tract or in ICC, however, has been characterized to a limited extent.
Knockouts of Ano1 – the gene precursor for ANO1 – in mouse models by classic
breeding schemes are typically fatal by Day 8, so a novel method of Ano1 elimination in
the GI tracts of fully developed mice would facilitate further characterization of its
function. In this study, inducible Cre-mediated excision resulted in significant Ano1
reduction in several GI tissues of adult mice, although significant knockdown was not
observed in the GI vasculature.
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In Copyright(All Rights Reserved)