Effect of curcumin on amyloid-like aggregates generated from methionine-oxidized apolipoprotein A-I
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Authors
Krishnamoorthy, Aparna
Tavoosi, Narjes
Chan, Gary K. L.
Liu, Jianfang
Ren, Gang
Cavigiolio, Giorgio
Ryan, Robert O.
Issue Date
2018
Type
Article
Language
Keywords
amyloid-like aggregate , Apolipoprotein A-I , curcumin , electron microscopy , fluorescence spectroscopy , methionine oxidation
Alternative Title
Abstract
Curcumin is a polyphenolic phytonutrient that has antineurodegenerative properties. In this study, we investigated the anti-amyloidogenic properties of curcumin. Following incubation with curcumin, intrinsic tryptophan fluorescence emission of apolipoprotein (apo) A-I was strongly quenched. At the same time, curcumin fluorescence emission was enhanced. The fluorescence emission spectra of curcumin in the presence of amyloid-like aggregates formed by methionine-oxidized (ox) apoA-I varied, depending on whether curcumin was added before, or after, aggregate formation. The impact of curcumin on the structure of the aggregating material was revealed by the lower amount of -structure in ox-apoA-I amyloid-like aggregates formed in the presence of curcumin, compared to aggregates formed without curcumin. However, the kinetics of ox-apoA-I amyloid-like aggregate formation was not altered by the presence of curcumin. Moreover, electron microscopy analysis detected no discernable differences in amyloid morphology when ox-apoA-I amyloid-like aggregates were formed in the presence or absence of curcumin. In conclusion, curcumin interacts with apoA-I and alters the structure of ox-apoA-I amyloid-like aggregates yet does not diminish the propensity of ox-apoA-I to form aggregates.
Description
Citation
Publisher
License
Attribution 4.0 International
Journal
Volume
Issue
PubMed ID
ISSN
2211-5463