High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia
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Authors
Vatter, Fanny A. Pelissier
Schapiro, Denis
Chang, Hang
Borowsky, Alexander D.
Lee, Jonathan K.
Parvin, Bahram
Stampfer, Martha R.
LaBarge, Mark A.
Bodenmiller, Bernd
Lorens, James B.
Issue Date
2018
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Article
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Abstract
Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. Aging human mammary tissue shows skewed progenitor cell potency, resulting in diminished tumor-suppressive cell types and the accumulation of defective epithelial progenitors. Quantitative characterization of these age-emergent human cell subpopulations is lacking, impeding our understanding of the relationship between age and cancer susceptibility. We conducted single-cell resolution proteomic phenotyping of healthy breast epithelia from 57 women, aged 16-91 years, using mass cytometry. Remarkable heterogeneity was quantified within the two mammary epithelial lineages. Population partitioning identified a subset of aberrant basal-like luminal cells that accumulate with age and originate from age-altered progenitors. Quantification of age-emergent phenotypes enabled robust classification of breast tissues by age in healthy women. This high-resolution mapping highlighted specific epithelial subpopulations that change with age in a manner consistent with increased susceptibility to breast cancer.
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Pelissier Vatter, F. A., Schapiro, D., Chang, H., Borowsky, A. D., Lee, J. K., Parvin, B., … Lorens, J. B. (2018). High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia. Cell Reports, 23(4), 1205–1219. doi:10.1016/j.celrep.2018.03.114
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2211-1247