The pineapple genome and the evolution of CAM photosynthesis

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Authors

Ming, Ray
VanBuren, Robert
Wai, Ching M.
Tang, Haibao
Schatz, Michael C.
Bowers, John E.
Lyons, Eric
Wang, Ming-Li
Chen, Jung
Biggers, Eric

Issue Date

2015

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Article

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HPC-Pronghorn

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Abstract

Pineapple (Ananas comosus (L.) Merr.) is the most economically valuable crop possessing crassulacean acid metabolism (CAM), a photosynthetic carbon assimilation pathway with high water-use efficiency, and the second most important tropical fruit. We sequenced the genomes of pineapple varieties F153 and MD2 and a wild pineapple relative, Ananas bracteatus accession CB5. The pineapple genome has one fewer ancient whole-genome duplication event than sequenced grass genomes and a conserved karyotype with seven chromosomes from before the. duplication event. The pineapple lineage has transitioned from C-3 photosynthesis to CAM, with CAM-related genes exhibiting a diel expression pattern in photosynthetic tissues. CAM pathway genes were enriched with cis-regulatory elements associated with the regulation of circadian clock genes, providing the first cis-regulatory link between CAM and circadian clock regulation. Pineapple CAM photosynthesis evolved by the reconfiguration of pathways in C-3 plants, through the regulatory neofunctionalization of preexisting genes and not through the acquisition of neofunctionalized genes via whole-genome or tandem gene duplication.

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Ming, R., VanBuren, R., Wai, C. M., Tang, H., Schatz, M. C., Bowers, J. E., … Biggers, E. (2015). The pineapple genome and the evolution of CAM photosynthesis. Nature Genetics, 47(12), 1435–1442. doi:10.1038/ng.3435

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1061-4036

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