Pervasive Genotypic Mosaicism in Founder Mice Derived from Genome Editing through Pronuclear Injection
Loading...
Authors
Oliver, Daniel K.
Yuan, Shuiqiao
McSwiggin, Hayden
Yan, Wei
Issue Date
2015
Type
Article
Language
Keywords
Alternative Title
Abstract
Genome editing technologies, especially the Cas9/CRISPR system, have revolutionized biomedical research over the past several years. Generation of novel alleles has been simplified to unprecedented levels, allowing for rapid expansion of available genetic tool kits for researchers. However, the issue of genotypic mosaicism has become evident, making stringent analyses of the penetrance of genome-edited alleles essential. Here, we report that founder mice, derived from pronuclear injection of ZFNs or a mix of guidance RNAs and Cas9 mRNAs, display consistent genotypic mosaicism for both deletion and insertion alleles. To identify founders with greater possibility of transmitting the mutant allele through the germline, we developed an effective germline genotyping method. The awareness of the inherent genotypic mosaicism issue with genome editing will allow for a more efficient implementation of the technologies, and the germline genotyping method will save valuable time and resources.
Description
Citation
Oliver, D., Yuan, S., McSwiggin, H., & Yan, W. (2015). Pervasive Genotypic Mosaicism in Founder Mice Derived from Genome Editing through Pronuclear Injection. PLOS ONE, 10(6), e0129457. doi:10.1371/journal.pone.0129457
Publisher
Journal
Volume
Issue
PubMed ID
ISSN
1932-6203