Elav-Mediated Exon Skipping and Alternative Polyadenylation of the Dscam1 Gene Are Required for Axon Outgrowth

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Authors

Zhang, Zhiping
So, Kevin
Peterson, Ryan
Bauer, Matthew
Ng, Henry
Zhang, Yong
Kim, Jung H.
Kidd, Thomas
Miura, Pedro

Issue Date

2019

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Article

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3? UTR , alternative cleavage and polyadenylation , alternative splicing , axon guidance , CRISPR , Dscam1 , Elav , exon skipping , MinION

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Abstract

Many metazoan genes express alternative long 3' UTR isoforms in the nervous system, but their functions remain largely unclear. In Drosophila melanogaster, the Dscam1 gene generates short and long (Dscam1-L) 3' UTR isoforms because of alternative polyadenylation (APA). Here, we found that the RNA-binding protein Embryonic Lethal Abnormal Visual System (Elav) impacts Dscaml biogenesis at two levels, including regulation of long 3' UTR biogenesis and skipping of an upstream exon (exon 19). MinION long-read sequencing confirmed the connectivity of this alternative splicing event to the long 3' UTR. Knockdown or CRISPR deletion of Dscam1-L impaired axon outgrowth in Drosophila. The Dscaml long 3' UTR was found to be required for correct Elav-mediated skipping of exon 19. Elav thus co-regulates APA and alternative splicing to generate specific Dscam1 transcripts that are essential for neural development. This coupling of APA to alternative splicing might represent a new class of regulated RNA processing.

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Zhang, Z., So, K., Peterson, R., Bauer, M., Ng, H., Zhang, Y., … Miura, P. (2019). Elav-Mediated Exon Skipping and Alternative Polyadenylation of the Dscam1 Gene Are Required for Axon Outgrowth. Cell Reports, 27(13), 3808�"3817.e7. doi:10.1016/j.celrep.2019.05.083

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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

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ISSN

2211-1247

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