Hormonally Modulated S-Nitrosation of Profilin-1 and Myosin Regulatory Light Polypeptide 9
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Authors
Tschernia, Andrew
Issue Date
2013
Type
Thesis
Language
en_US
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Abstract
The roles of estrogen and progesterone are highly regulated during the different
phases of pregnancy. The roles of these hormones and the cellular signal transduction of
labor contractions are well understood; however, the initiation of spontaneous preterm
labor contractions is still unknown in research today. The levels of estrogen and
progesterone are known to increase, and myosin regulatory light polypeptide 9 and
profilin-1 is known to be upregulated and S-nitrosated in preterm laboring myometrium.
We hypothesize that levels of estrogen and progesterone may have an effect on
posttranslational modification of myosin regulatory light polypeptide 9 and profilin-1.
Estrogen and Progesterone are highly regulated during the different phases of pregnancy.
Using western blot and biotin switch technique, we isolated S-nitrosated proteins, myosin
regulatory light polypeptide 9 and profilin-1, from hormonal and non hormonal growth
arrested cells lines of Pregnant Human Uterine Smooth Muscle Cell human Telomerase
Reverse Transcriptase (PHUSMC hTRT) for analysis. The results indicated that myosin
light polypeptide 9 had 1.45 and 1.68 fold increases (hormone and non-hormone) in Snitrosation.
Profilin-1 had 2.94 and 5.07 fold increases (hormone and non-hormone) S-nitrosation.
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